2 edition of Substitution of germanium for boron in suspension-cultured carrot cells found in the catalog.
Substitution of germanium for boron in suspension-cultured carrot cells
Robert W. Durst
Written in English
|Statement||by Robert W. Durst.|
|The Physical Object|
|Pagination||68 leaves, bound :|
|Number of Pages||68|
Cell division and the cell cycle are fundamental processes that are well, though not absolutely, conserved in all eukaryotes. Suspension cultured plant cells that can be synchronized offer the ideal experimental system in which to study the events and controls of the plant cell division cycle. Design, Synthesis, and Pharmacological Evaluation of 5,6-Disubstituted Pyridin-2(1H)-one Derivatives as Phosphodiesterase 10A (PDE10A) Antagonists.
The accurate, fast, and reliable determination of cell growth is of critical importance in plant cell and tissue culture However,"the"measurementof"growth"parameters"in"the". All sexually reproducing organisms produce primordial germ cells, a small population of cells that differentiate into gametes of either sex and carry to- potency, an ability to develop into an entire new organism. The study of germ cells has undergone enormous advances in recent years and has.
Carrot Cell Wall Chemistry Maturation of carrots resulted in an increase of fresh weight, dry weight and total root length (Ng et al., ). This was accompanied by an mcrease in cell-wall pectic Proceedings of the 7th International Worktng Conference on Stored-product Protection - Volume 2. Thieme E-Books & E-Journals. Abstract. The interest of biologists in boron (B) has largely been focused on its role in plants for which B was established as essential in (Warington, ).Evidence that B has a biological role in other organisms was first indicated by the establishment of essentiality of B for diatoms (Smyth and Dugger, ) and cyanobacteria (Bonilla et al.,
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To reevaluate this question, we have used suspension-cultured carrot cells and found that germanium can substitute for borate in this system. Germanate cultures were maintained by subculturing for more than two years.
Cell growth was studied as a function of borate or germanate concentration of the by: To\ud reevaluate this question, we have used\ud suspension-cultured carrot cells and found that germanium\ud can substitute for borate in this system.
Germanate\ud cultures were maintained by subculturing for more than\ud two years. Cell growth was studied as a function of\ud borate or germanate concentration of the medium. The possibility that Ge can substitute for B in plant growth and development has been a subject of debate (McIlrath and Skok, ; Loomis and Durst, ).
B-deficient carrot cells have been reported to grow albeit more slowly than B-sufficient cells, when Ge is Cited by: claimed that Ge does replace B because B-deficient suspension-cultured carrot (Daucus carota) cells grew, albeit at a slower rate than B-sufficient cells, when Ge was added to the culture medium.
Kobayashi et al. () have shown that in vitro, a RG-II dimer is formed when germanic acid is reacted with mono-meric RG-II (mRG-II).Cited by: the cell wall. The amounts of Ge in pumpkin leaves ( and ,ug Ge g-1 dry weight, 28 and lM Ge supply, respectively; Table I) are some-what higher than the amount of Ge (14 and tg Ge g-1 dry weight, 10 AM and 1 mm Ge supply, respectively) that is present in suspension-cultured carrot cells grown in the presence of Ge.
Boron (B)-deficient pumpkin (Cucurbita moschata Duchesne) plants exhibit reduced growth, and their tissues are brittle. The leaf cell walls of these plants contain less than one-half the amount of borate cross-linked rhamnogalacturonan II (RG-II) dimer than normal plants.
Supplying germanium (Ge), which has been reported to substitute for B, to B-deficient plants does not restore growth or. Durst RW, Loomis WD () Substitution of germanium for boron in suspension-cultured carrot cells. Plant Physiol 75S: 14 Google Scholar Edwards R, Dixon DP, Walbot V () Plant glutathione S -transferases: enzymes with multiple functions in sickness and in health.
Germanium Does Not Substitute for Boron in Cross-Linking of Rhamnogalacturonan II in Pumpkin Cell Walls Article (PDF Available) in Plant physiology (4) January with 37 Reads. Germanium can substitute for boron in carrot cell cultures.
Both boron and germanium are localized primarily in the cell wall. Boron is an essential element for higher plants, yet the primary functions remain unclear.
In intact tissues of higher plants, this element occurs as both water soluble and water insoluble forms. In this review, the intracellular localisation of B and possible function of B in cell walls of higher plants are discussed.
The majority of the water soluble B seems to be localised in the apoplastic. For this purpose, a suspension-cultured cell is an excellent model due to the elimination of inter-tissue and -organ communication such as carrot (Durst and Loomis ), tobacco (Matoh et al. ) Substitution of germanium for boron in suspension-cultured carrot cells.
Effect of germanium on utilization of boron in tomato (Lycopersion esculentum). Plant Substitution of germanium for boron in suspension cultured carrot cells. Plant Physiol. 14 Similarity in uptake and retention of trace amounts of silicon and germanium in rat tissues and cell organelles.
Bioinorg. Chem. 5: The aim of the present work was to investigate the involvement of ethylene together with lipid-derived factors and ROS in cell death response of BY-2 suspension cultured cells exposed to the chemical agents CdSO 4, ethylene, EtOH and MP. The experimental approach includes pharmacological analyses with the cell death inducers and inhibitors of.
The cells or tissues are obtained from any part of the plant like stem, root, leaf etc. which are encouraged to produce more cells in culture and to express their totipotency.
Cells or tissues are grown in different types of glass vials containing a medium with mineral nutrients, vitamins and phytohormones.
Cell Suspension Culture. 1. Structure Introduction. A generally accepted primary structure of pectin has emerged from cumulative studies spanning the last 70 years. The pectins used in these studies were typically isolated from plants of economic importance (e.g.
citrus, tomato, sugar beet, apple) or from suspension-cultured cells (e.g. sycamore, carrot, spinach, and rose). Suspension-cultured Chenopodium album L. cells are capable of continuous, long-term growth on a boron-deficient medium.
Compared with cultures grown with boron, these cultures contained more enlarged and detached cells, had increased turbidity due to the rupture of a small number of cells, and contained cells with an increased cell wall pore size. Subculture cells when they are in log-phase growth before they reach confluency.
When they reach confluency, cells in suspension clump together and the medium appears turbid when the culture flask is swirled. The maximum recommended cell density before passaging varies with cell lines; refer to the cell-specific product insert or manual for.
Germanium reportedly delays the symptoms of boron deficiency in plants and substitutes for boron in suspension-cultured carrot cells. However, germanate does not rescue the growth of boron-deficient pumpkin plants.
Virtually no germanate cross-linked RG-II dimer was detected even though germanate accumulated in the leaf cell walls. Take a small sample (μl) of the cells from the cell suspension and count the cells.
Calculate cells/ml and re-seed the desired number of cells into freshly prepared flasks, without centrifugation, just by diluting the cells.
Refer to the data sheet supplied with the cell line for the recommended seeding density. Repeat this every days. The most stable are formed with cis-diols on a furanoid ring. Two compounds have this structure physiologically: ribose in ribonucleotides and RNA, and apiose in the plant cell wall.
Germanium can substitute for boron in carrot cell cultures. Both boron and germanium are localized primarily in the cell wall. We postulate that borate-apiofuranose ester cross-links are the auxin-sensitive acid.
Robert W. Durst has written: 'Substitution of germanium for boron in suspension-cultured carrot cells' -- subject(s): Boron, Effect of boron on Plants, Germanium, Physiological effect, Plants.suspension cells.
Growth pattern of the suspension cells were studied in full and half strength MS media. Cells were harvested three times in at 5 days interval upto 15 days. The highest cell growth obtained was on the 15th day in full strength MS medium.
Analysis of secondary metabolites such as.The ﬁrst commercially interesting suspension cell products [food-and-mouth disease vaccine produced in suspension culture of BHK cells and interferon produced in Namalva cells (5)] stimulated the adaptation of homogeneous bioreactor systems used for microbial culture to the requirements of the mechanically more sensitive animal cells.